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Primary liver cancer is one of the common malignant tumors.However, the efficacy of traditional treatments is not satisfactory.RNA interference is a new treatment method, which develops rapidly in recent years.It has been found that RNA interference can exert the anti-tumor effect through inhibiting the growth and proliferation of hepatocarcinoma cells, promoting apoptosis, inhibiting the invasion and metastasis of hepatocarcinoma cells, inhibiting angiogenesis and overcoming the resistance to chemotherapy.
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Aims To analyze the intervention effect of Captopril on serum endogenous metabolites alternations in spontaneous hypertension rats ( SHR) and to investi-gate possible therapeutic mechanism .Methods The rapid resolution liquid chromatography/quadrupole-time of flight tandem mass spectrometry ( RRLC-Q-TOF-MS) and technology coupled with partial least squares discriminant analysis ( PLS-DA ) processed by SIMCA-P software were used to distinguish significantly different variables and identify potential biomarkers . Results Compared to the normal group , metabolic profiling changed significantly in model group and Cap-toril group .Totally 4 metabolins and their metabolic pathways were detected , which were closely related to the endothelial function .Conclusion Metabolomics reveals possible therapeutic mechanism of Captopril for protecting endothelial function from overall metabolism of the body .It also shows unique potential in terms of interpretation of the complex mechanisms of drugs .
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Objective To discuss the medication principles of diagnosis and treatment for insomnia from spleen and stomach; To provide references for clinical treatment for insomnia.Methods Relevant articles about diagnosis and treatment forinsomnia from spleen and stomach were searched in CNKI and Wanfang Data through computers, the data were input into Traditional Chinese Medicine Inheritance Supporting System (V2.5) and then association rules, apriori arithmetic, improved mutual information method, and complex system entropy cluster methods were used to analyze the medication principles of diagnosis and treatment for insomnia from spleen and stomach. Results Through screening, totally 87 prescriptions were included, involving 165 herbal medicine. Data mining results showed that there were 30 high-frequency herbal medicine, 19 high-frequency medicinal combinations, and 4 medicinal association rules. Evolution led to 13 medicinal combinations of 2 herbal medicine and 3 new prescriptions. Conclusion The main method of diagnosis and treatment for insomnia from the spleen and stomach is regulating qi activity of spleen and stomach. The features of common medicine are invigorating spleen to reduce phlegm, clearing stomach and calming the adverse-rising energy, and tonifying qi and soothing nerves, which can offer references for clinical treatment for insomnia.
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Objective To observe the changes of serum endotoxin and interleukin-6 level before and after treatment at intestine get rid of filth and adjustment intestine microenviroment in patients with advanced stage liver cirrhosis and to oberserve the incidence of spontaneous bacterial peritonitis(SBP) and to research a method that can more efficient reduce the incidence of SBP.Methods Group A was given conventional treatment such as protection liver,supplement human albumin and uragogue;goup B was given FPA 0 2g tid po,continuous three days on the basis of two weeks conventional treatment,group C was given Tab comfort get 0 5g tid po,Changtai orally taken liquid,10ml tid po,lactulose 10ml tid po, for all 8 weeks,on the basis of group B treatment after pausing use FPA.Results Serum endotoxin and interleukin-6 levels in group A was not significantly changes after treatment,,SBP incidence was higher than both group B and group C;at group B,serum endotoxin and interleukin-6 levels decline,SBP incidence significantly drop afer treatment,at group C,serum endotoxin,interleukin-6 level and SBP incidence significantly drop,the difference was significant at statistics as compared with group A and B.Conclusions The treatment at intestine get rid of filth and adjustment intestine microenviroment can effectively reduce serum endotoxin and interleukin-6 levels and can significantly reduce the incidence of SBP.
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AIM: To study the effects of spirulina (Spirin) in the prevention of liver fibrosis in rats. METHODS: Wistar rats were divided into three groups: control group(n=8); CCl4 group(n=8), spirulina group(n=8). CCl4 group and spirulina group rats were induced by 40% CCl4 olive oil solution into models of liver fibrosis, meanwhile spirulina group was given the feed with spirulina powder for 12 wk. Liver histological changes, hydroxyproline(HYP) content in liver tissues, serum procollagen type Ⅲ(PC-Ⅲ) and hyaluronic acid(HA) levels were observed. RESULTS: The pathological studies showed that the liver fibrosis degree of spirulina group was obviously lighter than that of CCl4 group. Spirulina group had significantly lower levels of HYP(174 μg*g-1±37 μg*g-1), PC-Ⅲ(140 μg*L-1±37 μg*L-1) and HA(262 μg*L-1±92 μg*L-1) than those of CCl4 group (243 μg*g-1±49 μg*g-1, 274 μg*L-1±69 μg*L-1 and 484 μg*L-1±114 μg*L-1, respectively). CONCLUSION: The experimental evidence suggests that spirulina can prevent the formation of liver fibrosis.
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Objective To investigate the relationship between the serum c oncentration of TGF-? 1 and invasion and metastasis of gastric cancer,and the effect of surgical treatment on the serum concentration of TGF-? 1.M ethods The serum concentration of TGF-? 1 of 48 cases of gastric can cer was detected by ELISA 3 days before and 7-10 days after surgical treatment. Results The serum concentration of TGF-? 1 of gastric cancer before surgical treatment (404.6? 12.0) mg/L was higher than that after surg ical treatment (294.3?33.9) mg/L (P